Showing posts from August, 2014

Install a Blast and download a database in Ubuntu!

First install Blast sudo apt-get install blast2 Make a blast Directory
mkdir /home/username/blast/db cd /home/username/blast/db connect to blast FTP
ftp Connected to 220- Warning Notice! [ ... Extra warning message removed for brevity ... ] Name ( anonymous 331 Anonymous login ok, send your complete email address as your password. Password: [note: enter your email address at this prompt] 230-Anonymous access granted, restrictions apply. Please read the file README.ftp 230 it was last modified on Fri Mar 28 14:05:45 2008 - 716 days ago Remote system type is UNIX. Using binary mode to transfer files. Enter Blast database directory
ftp> cd blast/db Get a database. Below is an example and exit ftp
ftp> get 16SMicrobial.tar.gz ftp> bye 221 Goodbye. Extract the database
tar zxvpf 16SMicrobial.tar.gz Enter your query sequence in a file (q16s.txt) and execute the command below.
blastall -p blastn -i …